Coating Titania Nanoparticles with Epoxy-Containing Catechol Polymers via Cu(0)-Living Radical Polymerization as Intelligent Enzyme Carriers.
Donghao WangWenyi DingKaiyue ZhouShutong GuoQiang ZhangDavid M HaddletonPublished in: Biomacromolecules (2018)
Immobilization of enzyme could offer the biocatalyst with increased stability and important recoverability, which plays a vital role in the enzyme's industrial applications. In this study, we present a new strategy to build an intelligent enzyme carrier by coating titania nanoparticles with thermoresponsive epoxy-functionalized polymers. Zero-valent copper-mediated living radical polymerization (Cu(0)-LRP) was utilized herein to copolymerize N-isopropylacrylamide (NIPAM) and glycidyl acrylate (GA) directly from an unprotected dopamine-functionalized initiator to obtain an epoxy-containing polymer with terminal anchor for the "grafting to" or "one-pot" modification of titania nanoparticles. A rhodamine B-labeled laccase has been subsequently used as a model enzyme for successful immobilization to yield an intelligent titania/laccase hybrid bifunctional catalyst. The immobilized laccase has shown excellent thermal stability under ambient or even relatively high temperature above the lower critical solution temperature (LCST) at which temperature the hybrid particles could be facilely recovered for reuse. The enzyme activity could be maintained during the repeated use after recovery and enzymatic degradation of bisphenol A was proven to be efficient. The photocatalytic ability of titania was also investigated by fast degradation of rhodamine B under the excitation of simulated sunlight. Therefore, this study has provided a facile strategy for the immobilization of metal oxide catalysts with enzymes, which constructs a novel bifunctional catalyst that will be promising for the "one-pot" degradation of different organic pollutants.
Keyphrases
- highly efficient
- metal organic framework
- reduced graphene oxide
- quantum dots
- ionic liquid
- high temperature
- wastewater treatment
- room temperature
- fluorescent probe
- heavy metals
- gold nanoparticles
- hydrogen peroxide
- risk assessment
- magnetic nanoparticles
- mass spectrometry
- nitric oxide
- metabolic syndrome
- uric acid
- carbon dioxide
- pet imaging
- liquid chromatography
- oxide nanoparticles