Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates.
Thomas BoehmMatthias KarerElisabeth GludovaczKarin PetrocziMarlene ReschKornelia SchuetzenbergerKristaps KlavinsNicole BorthBernd JilmaPublished in: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (2020)
Incubation of DAO with the natural substrates putrescine and cadaverine and oABA generates novel fluorophores increasing the detection limit compared to absorption measurements at least tenfold. This simple, sensitive and specific assay allows the non-radioactive quantification of DAO activity in complex matrices like plasma and tissue extracts without interference by antioxidants.