Detection of Leptospira species in bat cadavers, Czech and Slovak Republics.

Veronika SeidlovaPetra StrakováRomana KejíkováMonika NemcovaTomáš BartoničkaJiří SalátLucie DufkováSilvie ŠikutováJan MendelClifton McKeeJan Zukal Jiri Pikula Ivo Rudolf

Published in: Emerging microbes & infections (2022)

Kidney samples from 300 bat cadavers from the Czech and Slovak Republics were tested for Leptospira DNA using PCR and sequencing of three genes ( lipL32 , flab , and 16S ribosomal RNA). Overall detection rate was 4.7% and two bat species ( Myotis myotis and Nyctalus noctula ) were PCR-positive for at least one gene. Detected Leptospira sequences were similar to L. interrogans and L. borgpetersenii , and included a potentially novel species related to L. weilii .

Keyphrases

real time pcr
genome wide
genetic diversity
loop mediated isothermal amplification
label free
genome wide identification
single molecule
gene expression
nucleic acid
dna methylation
transcription factor