Luminescence detection of peptide: N -glycanase activity using engineered split inteins.

A split intein-based method has been developed to detect peptide: N -glycanase (PNGase) activity in live cells. PNGase cleaves the linkage between N,N '-diacetylchitobiose and the Asn side-chain of N-intein peptides and the products react rapidly with C-intein by protein trans-splicing to generate an active luciferase.