Fluorescence in situ hybridization and polymerase chain reaction to detect infections of cardiac implantable electronic devices.
Isabell Anna JustFrank BarthelAnnette MoterJudith KikhneyAljona FriedrichAlexa WlochVolkmar FalkChristoph T StarckFelix SchoenrathPublished in: Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology (2022)
Fluorescence in situ hybridization sequencing is a valuable tool to detect causative microorganisms in CIED infections. The combination of FISHseq with preoperative cultivation showed the highest efficacy in detecting pathogens. Additional cultivation of intraoperative tissue samples or swabs yielded more confirmation of pathogens known from preoperative culture.