Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast.
Lihui JiangXiaoyan ZhangYiting ZhaoHaiyan ZhuQijing FuXinqi LuWuying HuangXinyue YangXuan ZhouLixia WuAo YangXie HeMan DongZiai PengJing YangLiwei GuoJiancheng WenHuichuan HuangYong XieShusheng ZhuChengyun LiXiahong HeYouyong ZhuJiří FrimlYunlong DuPublished in: Nature communications (2024)
Phytoalexin sakuranetin functions in resistance against rice blast. However, the mechanisms underlying the effects of sakuranetin remains elusive. Here, we report that rice lines expressing resistance (R) genes were found to contain high levels of sakuranetin, which correlates with attenuated endocytic trafficking of plasma membrane (PM) proteins. Exogenous and endogenous sakuranetin attenuates the endocytosis of various PM proteins and the fungal effector PWL2. Moreover, accumulation of the avirulence protein AvrCO39, resulting from uptake into rice cells by Magnaporthe oryzae, was reduced following treatment with sakuranetin. Pharmacological manipulation of clathrin-mediated endocytic (CME) suggests that this pathway is targeted by sakuranetin. Indeed, attenuation of CME by sakuranetin is sufficient to convey resistance against rice blast. Our data reveals a mechanism of rice against M. oryzae by increasing sakuranetin levels and repressing the CME of pathogen effectors, which is distinct from the action of many R genes that mainly function by modulating transcription.
Keyphrases
- air pollution
- particulate matter
- genome wide
- induced apoptosis
- drug delivery
- oxidative stress
- machine learning
- cell proliferation
- transcription factor
- electronic health record
- immune response
- gene expression
- risk assessment
- cell death
- cancer therapy
- polycyclic aromatic hydrocarbons
- bioinformatics analysis
- endoplasmic reticulum stress
- protein protein