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Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach.

Maria QuitérioSandra SimõesAndreia AscensoManuela Colla CarvalheiroAntónio J AlmeidaIsabel CorreiaAna Silveira VianaPedro FaíscaLia AscensãoJesús MolpeceresMaria Manuela GasparCatarina Pinto Reis
Published in: International journal of molecular sciences (2021)
Insulin is a peptide hormone with many physiological functions, besides its use in diabetes treatment. An important role of insulin is related to the wound healing process-however, insulin itself is too sensitive to the external environment requiring the protective of a nanocarrier. Polymer-based nanoparticles can protect, deliver, and retain the protein in the target area. This study aims to produce and characterize a topical treatment for wound healing consisting of insulin-loaded poly-DL-lactide/glycolide (PLGA) nanoparticles. Insulin-loaded nanoparticles present a mean size of approximately 500 nm and neutral surface charge. Spherical shaped nanoparticles are observed by scanning electron microscopy and confirmed by atomic force microscopy. SDS-PAGE and circular dichroism analysis demonstrated that insulin preserved its integrity and secondary structure after the encapsulation process. In vitro release studies suggested a controlled release profile. Safety of the formulation was confirmed using cell lines, and cell viability was concentration and time-dependent. Preliminary safety in vivo assays also revealed promising results.
Keyphrases
  • wound healing
  • type diabetes
  • glycemic control
  • drug delivery
  • atomic force microscopy
  • cancer therapy
  • metabolic syndrome
  • single molecule
  • high resolution
  • combination therapy
  • single cell
  • smoking cessation