Biocatalytic Hybrid Films Self-Assembled from Carbohydrate Block Copolymers and Polysaccharides for Enzyme Prodrug Therapy.

Biocatalytic films are attracting growing attention for their significant potential as scaffolds for therapeutic reactor devices. However, conventional film fabrication methods result either in enzyme denaturation or require cumbersome procedures. Here, we report the preparation of biocatalytic films via self-assembly of a carbohydrate block copolymer and a polysaccharide. Enzyme-loaded films can be prepared by simply drying the polymer solution, and the loaded enzymes retain their biocatalytic activities in the film for prolonged periods of time. We also demonstrate that the enzyme-loaded films can successfully transform a prodrug into an antitumor drug that inhibits tumor cell growth. Our work highlights the potential of these biocatalytic self-assembled films as therapeutic reactor devices for enzyme prodrug therapy. Given the simplicity of the preparation method, this approach could improve the versatility of biocatalytic films and consequently expand their applicability from exclusive use in therapeutic reactor devices to sensing and diagnosis.