Natural versus Recombinant Viral Antigens in SARS-CoV-2 Serology: Challenges in Optimizing Laboratory Diagnosis of COVID-19.
Luciana Regina MeirelesAngélica Moura Freixeira da SilvaCamila Aparecida de CarvalhoNorival KesperAndrés Jimenez GalisteoCamila Pereira SoaresDanielle Bastos AraujoEdison Luiz DurigonDanielle Bruna Leal de OliveiraLigia MorgantiRosa Maria Chura-ChambiHeitor Franco de Andrade JuniorPublished in: Clinics (Sao Paulo, Brazil) (2020)
The choice of antigen is an important aspect in optimizing the laboratory diagnosis of COVID-19. The use of rNP ELISA for the detection of anti-SARS-CoV-2 IgG antibodies seems promising, but comparison of the results with those of WVA ELISA is crucial for accurate test development prior to commercialization. IgG serology using several assays, and with the spectral patterns of SARS-CoV-2, resulted in confusing information that must be clarified before the establishment of diagnostic serology criteria.