In vitro evaluation of the activity of Cinnamaldehyde as an inhibitor of the biological cycle of Ctenocephalides felis felis .

The objective of this work was to evaluate the in vitro activity of cinnamaldehyde as an inhibitor of the biological cycle of Ctenocephalides felis felis . Tests were carried out with six repetitions containing 10 eggs of C. felis felis together with 0.5g of diet and filter paper discs with area of 23.76 cm 2 impregnated with different concentrations of cinnamaldehyde (6; 8; 10; 12; 14; 16; 18; 20; 24 and 28 µg.cm -2 ) in Petri dishes and incubated in a climate-controlled chamber at 27 ± 1 °C and RH 75 ± 10%, for 30 days. The tests included a placebo group containing the diluent (acetone) and a negative control group, without treatment. At the end of 30 days, the percentage of inhibition of adult emergence/mortality was calculated, as well as the LC 50 and LC 90 . Inhibition of adult emergence greater than 50% was observed from a concentration of 10 µg.cm -2 and 100% inhibition from a concentration of 14 µg.cm -2 , obtaining LC 50 and LC 90 results of 8.75 and 13.57 µg.cm -2 , respectively. We concluded that the volatile compound cinnamaldehyde is effective in vitro as an inhibitor of the biological cycle of C. felis felis .