The Outcome of Ex Vivo TIL Expansion Is Highly Influenced by Spatial Heterogeneity of the Tumor T-Cell Repertoire and Differences in Intrinsic In Vitro Growth Capacity between T-Cell Clones.
Isabel C PoschkeJessica C HasselAaron Rodriguez-EhrenfriedKatharina A M LindnerIgnacio Heras-MurilloLena M AppelJohanna LehmannTanja LövgrenStina L WickströmClaudia LauensteinJasmin RothAnna-Katharina KönigJohn B A G HaanenJoost van den BergRolf KiesslingFrank BergmannMichael FlossdorfOliver StrobelRienk OffringaPublished in: Clinical cancer research : an official journal of the American Association for Cancer Research (2020)
Culture-induced changes in clonal composition are likely to affect tumor reactivity of TIL preparations. TCR deep sequencing provides important insights into the factors that govern the outcome of in vitro TIL expansion and thereby a path toward optimization of the production of TIL preparations with high therapeutic efficacy.See related commentary by Lozano-Rabella and Gros, p. 4177.
Keyphrases