Integrating pathway elucidation with yeast engineering to produce polpunonic acid the precursor of the anti-obesity agent celastrol.
Nikolaj L HansenKarel MiettinenYong ZhaoCodruta IgneaAggeliki AndreadelliMorten H RaadamAntonios M MakrisBirger L MøllerDan StærkSøren BakSotirios C KampranisPublished in: Microbial cell factories (2020)
Our study elucidates the early steps of celastrol biosynthesis and paves the way for future biotechnological production of this pharmacologically promising compound in engineered yeast strains.