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Establishment of Induced Pancreatic Stem Cells by Yes-Associated Protein 1.

Hirofumi NoguchiChika Miyagi-ShiohiraYoshiki NakashimaYuka OnishiIssei SaitohMasami Watanabe
Published in: Cell transplantation (2024)
Recently, we and others generated induced tissue-specific stem/progenitor (iTS/iTP) cells. The advantages of iTS/iTP cells compared with induced pluripotent stem (iPS) cells are (1) easier generation, (2) efficient differentiation, and (3) no teratomas formation. In this study, we generated mouse induced pancreatic stem cells (iTS-P cells) by the plasmid vector expressing Yes-associated protein 1 ( YAP ). The iTS-P YAP9 cells expressed Foxa2 (endoderm marker) and Pdx1 (pancreatic marker) while the expressions of Oct3/4 and Nanog (marker of embryonic stem [ES] cells) in iTS-P YAP9 cells was significantly lower compared with those in ES cells. The iTS-P YAP9 cells efficiently differentiated into insulin-expressing cells compared with ES cells. The ability to generate autologous iTS cells may be applied to diverse applications of regenerative medicine.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • endoplasmic reticulum stress
  • signaling pathway
  • escherichia coli
  • bone marrow
  • crispr cas
  • endothelial cells
  • optical coherence tomography
  • high glucose
  • pi k akt
  • diabetic rats