Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study.

Janis M TaubeKristin RomanElizabeth L EngleChichung WangCarmen Ballesteros-MerinoShawn M JensenJohn McGuireMei JiangCarla ColtharpBethany RemeniukIgnacio WistubaDarren LockeEdwin R ParraBernard A FoxDavid L RimmCliff Hoyt

Published in: Journal for immunotherapy of cancer (2022)

Assay optimization yielded highly sensitive, reproducible mIF characterization of the PD-1/PD-L1 axis across multiple sites. High concordance was observed across sites for measures of density of specific IC subsets, measures of coexpression and proximity with single-cell resolution.

Keyphrases

single cell
high throughput
rna seq
mass spectrometry
high resolution