Development of a Trypanosoma cruzi strain typing assay using MS2 peptide spectral libraries (Tc-STAMS2).
Gilberto Santos de OliveiraRebeca KawaharaLivia Rosa-FernandesSimon Ngao MuleCarla Cristi AvilaMarta M G TeixeiraMartin R LarsenGiuseppe PalmisanoPublished in: PLoS neglected tropical diseases (2018)
This is the first study that develops a MS-based platform for T. cruzi strain typing. Indeed, the Tc-STAMS2 method allows T. cruzi strain typing using MS/MS spectra as discriminatory features and allows the differentiation of TcI-TcVI DTUs. Similar to genomic-based strategies, the Tc-STAMS2 method allows identification of strains within DTUs. Its robustness towards different experimental and biological variables makes it a valuable complementary strategy to the current T. cruzi genotyping assays. Moreover, this method can be used to identify DTU-specific features correlated with the strain phenotype.
Keyphrases
- ms ms
- high throughput
- mass spectrometry
- multiple sclerosis
- genetic diversity
- trypanosoma cruzi
- escherichia coli
- genome wide
- liquid chromatography tandem mass spectrometry
- gene expression
- optical coherence tomography
- magnetic resonance
- density functional theory
- high performance liquid chromatography
- bioinformatics analysis