Characterization of Port Bolivar Virus, a Novel Entomobirnavirus (Birnaviridae) Isolated from Mosquitoes Collected in East Texas, USA.

Robert B TeshBethany G BollingHilda GuzmanVsevolod L PopovAshley WilsonSteven G WidenThomas G WoodPeter J Walker Nikos Vasilakis

Published in: Viruses (2020)

This report describes and characterizes a novel entomobirnavirus, designated Port Bolivar virus (PTBV), that was isolated from a pool of Aedes sollicitans mosquitoes collected in a saltwater marsh in East Texas, USA. Full genome sequencing and phylogenetic analyses indicate that PTBV is distinct but genetically related to Drosophila X virus and mosquito X virus, which are assigned to species in the genus Entomobirnavirus, family Birnaviridae. PTBV produced cytopathic effect (CPE) in cultures of mosquito (C6/36) cells, but not in Vero cell cultures. Ultrastructural studies of PTBV in infected C6/36 cells demonstrated unenveloped virus particles about 55 nm in diameter.

Keyphrases

aedes aegypti
dengue virus
induced apoptosis
cell cycle arrest
single cell
gene expression
disease virus
genome wide
stem cells
cell death
bone marrow