Selective Spectrofluorimetric Approach for the Assessment of Two Antipsychotic Drugs through Derivatization with O -Phthalaldehyde.
Hassan M A HassanIbrahim Hotan AlsohaimiMohamed Y El-SayedIsmail I AliAkram M El-DidamonyHamud A AltalebMutairah S AlshammariPublished in: Pharmaceuticals (Basel, Switzerland) (2022)
A facile and sensible spectrofluorimetric approach for the measurement of two antipsychotic medications, aripiperazole (ARP) and clozapine (CLZ), was devised and validated. The approach involves reacting the examined medicines with o -phthalaldehyde in the presence of β-mercaptoethanol in a borate buffer of pH 9.0 and pH 10 for ARP and CLZ, respectively, to produce a robustly fluorescent compound that is detected at 450 nm following excitation at 340 nm. The experimental variables influencing the performance and product stability were thoroughly investigated and optimized. Under optimal conditions, the intensity of the fluorescence was linear during a concentration range of 0.1-0.5 μg/mL, with a limit of detection (0.0391 and 0.0400 μg/mL) and limit of quantitation (0.1035 and 0.1332 μg/mL), respectively, for ARP and CLZ. The suggested approach was successful in analyzing commercialized tablets. A statistical investigation of the results produced by the suggested and standard methods showed no remarkable variation in the precision and accuracy of the two approaches. A chemical mechanism using o -phthalaldehyde was proposed.
Keyphrases
- ms ms
- quantum dots
- liquid chromatography tandem mass spectrometry
- photodynamic therapy
- high performance liquid chromatography
- energy transfer
- liquid chromatography
- label free
- mass spectrometry
- tandem mass spectrometry
- solid phase extraction
- single molecule
- simultaneous determination
- gas chromatography mass spectrometry
- living cells
- atomic force microscopy
- high intensity
- loop mediated isothermal amplification
- drug induced