A dual-channel Hill-type small-molecule pH probe.

Fang HuYunxia HuangYansheng XiaoYanchun LiXiao LuoXuhong QianYoujun Yang

Published in: Analytical methods : advancing methods and applications (2021)

By combining a Hill-type pH probe and a pH-insensitive naphthalimide fluorophore, we synthesized a FRET-based ratiometric pH probe (PHHF), exhibiting a reduced pH transition width, representing a unique approach for development of sensitive probes for detection of biorelevant pH changes.

Keyphrases

living cells
fluorescent probe
small molecule
quantum dots
single molecule
sensitive detection
hydrogen peroxide