Whole Genome Sequencing and Comparative Genomics of Six Staphylococcus schleiferi and Staphylococcus coagulans Isolates.
Mohamed A AbouelkhairStephen A KaniaPublished in: Genes (2024)
Staphylococcus schleiferi and Staphylococcus coagulans , closely related bacterial species within the Staphylococcus genus, present a challenge in classification and diagnosis due to their close genetic proximity and overlapping phenotypic features. Moreover, our understanding of the virulence mechanisms in staphylococcal species, beyond the extensively studied Staphylococcus aureus , remains limited, underscoring the importance of using comparative data to enhance our insights into virulence within these bacterial species. This study employed a comprehensive approach, utilizing comparative genomics, to identify genomic distinctions between S. schleiferi and S. coagulans , aiming to address the challenges in the accurate classification and diagnosis of these organisms and identify unique features. Whole genome sequencing was performed on six clinical isolates, and their genomes were compared to identify variations in gene content and virulence factors. De novo assembly and annotation revealed two samples as S. coagulans and four samples as S. schleiferi . Analysis of the core genomes revealed conserved regions crucial for defining species identity, while accessory genomic elements contained unique genes, possibly impacting the pathogenicity of the species.
Keyphrases
- staphylococcus aureus
- biofilm formation
- pseudomonas aeruginosa
- methicillin resistant staphylococcus aureus
- genetic diversity
- single cell
- escherichia coli
- genome wide
- copy number
- candida albicans
- machine learning
- deep learning
- dna methylation
- cystic fibrosis
- rna seq
- gene expression
- artificial intelligence
- antimicrobial resistance
- drug induced
- big data
- mass spectrometry