Functionalization of Gold Nanostars with Melamine for Colorimetric Detection of Uric Acid.
Sai Snigdha DanduDharaben J JoshiTae Jung ParkSuresh Kumar KailasaPublished in: Applied spectroscopy (2023)
Gold nanostars (AuNSs) are synthesized using a seed-mediated growth method. The synthesized AuNSs solution is stable and shows a localized surface plasmon resonance (LSPR) band in the visible range, which is confirmed using ultraviolet-visible (UV-Vis) spectroscopy. Furthermore, the as-synthesized AuNSs were functionalized with melamine and used as a sensor for the colorimetric detection of uric acid (UA). The detection mechanism could be assessed through various analytical techniques such as UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, dynamic light scattering (DLS), zeta potential, field emission scanning electron microscopy (FE-SEM), and transmission electron microscopic techniques. These methods exhibited a good linear regression between the absorption ratio of LSPR band of melamine-AuNSs and the concentration of UA (0-120 µM), with the detection limit of 8.50 nm. As a result, UA was quantitatively detected in biofluids by using melamine-AuNSs as a colorimetric sensor, revealing melamine-AuNSs-based colorimetric approach which could be used as a simple platform for UA assay in biofluids.
Keyphrases
- uric acid
- gold nanoparticles
- label free
- electron microscopy
- loop mediated isothermal amplification
- molecularly imprinted
- sensitive detection
- metabolic syndrome
- hydrogen peroxide
- high resolution
- aqueous solution
- fluorescent probe
- real time pcr
- living cells
- single molecule
- risk assessment
- quantum dots
- nitric oxide
- mass spectrometry
- climate change
- human health
- liquid chromatography