Cucurbitacin E Inhibits Huh7 Hepatoma Carcinoma Cell Proliferation and Metastasis via Suppressing MAPKs and JAK/STAT3 Pathways.
Yang LiuHeng YangQiang GuoTingting LiuYong JiangMingbo ZhaoKewu ZengPengfei TuPublished in: Molecules (Basel, Switzerland) (2020)
Cucurbitacin E (CuE), a highly oxygenated tetracyclic triterpene from Cucurbitaceae, has shown to exhibit potent cytotoxic and anti-proliferative properties against several human cancer cells. However, the underlying effects and mechanisms of CuE regarding hepatocellular carcinoma (HCC) have not been well understood. In the current study, unbiased RNA-sequencing (RNA-seq) and bioinformatics analysis was applied to elucidate the underlying molecular mechanism. CuE could significantly inhibit cell proliferation and migration of Huh7 cells, meanwhile CuE exhibited potent anti-angiogenic activity. RNA-seq analysis revealed that CuE negatively regulated 241 differentially expressed genes (DEGs) involved in multiple processes including cytoskeleton formation, angiogenesis and focal adhesion. Further analysis revealed that CuE effectually regulated diversified pharmacological signaling pathways such as MAPKs and JAK-STAT3. Our findings demonstrated the role of CuE in inhibiting proliferation and migration, providing an insight into the regulation of multiple signaling pathways as a new paradigm for anti-cancer treatment strategy.
Keyphrases
- single cell
- rna seq
- signaling pathway
- induced apoptosis
- bioinformatics analysis
- cell proliferation
- endothelial cells
- transcription factor
- pi k akt
- gene expression
- epithelial mesenchymal transition
- oxidative stress
- cell death
- mesenchymal stem cells
- genome wide
- vascular endothelial growth factor
- cell cycle
- endoplasmic reticulum stress
- cystic fibrosis
- pseudomonas aeruginosa
- induced pluripotent stem cells