Generation of sheep with defined FecB B and TBXT mutations and porcine blastocysts with KCNJ5 G151R/+ mutation using prime editing.

Our study demonstrates the potential of the PE system to edit the genomes of large animals for the induction of economically desired mutations and for modeling human diseases. Although prime-edited sheep and porcine blastocysts could be generated, the editing frequencies are still unsatisfactory, highlighting the need for optimizations in the PE system for efficient generation of large animals with customized traits.