A lipophilic copper(ii) complex as an optical probe for intracellular detection of NO.
Neil WilsonLok Hang MakAgostino CilibrizziAntony D GeeNicholas J LongRudiger WoscholskiRamon VilarPublished in: Dalton transactions (Cambridge, England : 2003) (2018)
A new chemical sensor for cellular imaging of NO is presented. This cell-permeable probe is based on a complex where copper(ii) is coordinated to a tridentate ligand substituted with a fluorophore (NBD) and an octyl group. The fluorescence response of this complex towards a range of reactive species (namely NO, NO2-, NO3-, H2O2, ClO-, O2- and ONOO-) has been studied in vitro showing that the probe is highly selective for NO. The probe is readily taken up by cells and is able to image the cellular concentrations of NO.
Keyphrases
- living cells
- quantum dots
- fluorescent probe
- high resolution
- induced apoptosis
- single molecule
- deep learning
- cell cycle arrest
- single cell
- molecular docking
- machine learning
- signaling pathway
- endoplasmic reticulum stress
- cell proliferation
- oxide nanoparticles
- photodynamic therapy
- high speed
- loop mediated isothermal amplification
- reactive oxygen species
- energy transfer
- mesenchymal stem cells
- fluorescence imaging