Cloning and promoter analysis of palladin 90-kDa, 140-kDa, and 200-kDa isoforms involved in skeletal muscle cell maturation.
Boimpoundi Eunice Flavie OualiTzu-Yu LiuChun-Yen LuPei-Yuan ChengChao-Li HuangChun-Chun LiYu-Chung ChiangHao-Ven WangPublished in: BMC research notes (2020)
By using promoter prediction programs, we successfully identified the transcription start sites for the Palld isoforms and revealed the presence of a variety of transcriptional regulatory elements including TATA box, GATA, MyoD, myogenin, MEF, Nkx2-5, and Tcf3 upstream promoter regions. The transcriptome profiling approach confirmed the active role of predicted transcription factors in the mouse genome. This study complements the missing piece in the characterization of palladin gene and certainly contributes to understanding the complexity and enrollment of palladin regulatory factors in gene transcription.
Keyphrases
- transcription factor
- genome wide identification
- single cell
- genome wide
- heat shock protein
- skeletal muscle
- rna seq
- dna binding
- dna methylation
- copy number
- gene expression
- public health
- insulin resistance
- cell therapy
- healthcare
- health insurance
- stem cells
- metabolic syndrome
- adipose tissue
- oxidative stress
- heat shock
- heat stress