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Establishment and evaluation of an indirect immunofluorescence assay for the detection of salmonid alphavirus.

S GaoW ShiY T WangM T GuoK X DuanA C SongG H LianT RenY J LiLijie TangL SunM Liu
Published in: Letters in applied microbiology (2018)
In this study, we successfully prepared two monoclonal antibodies (mAbs) against three subtypes of salmonid alphavirus (SAV), and established an indirect immunofluorescence assay that can be used to diagnose and prevent SAV from entering SAV unreported countries, such as China, through international trade. The application of this method will contribute to controlling SAV infection and reducing economic losses.
Keyphrases
  • high throughput
  • loop mediated isothermal amplification
  • quantum dots
  • sensitive detection