Understanding the Potential In Vitro Modes of Action of Bis(β-diketonato) Oxovanadium(IV) Complexes.
Baris SergiIpek BulutYing XiaZoë A E WallerYasemin YildizhanCeyda AcilanRianne M LordPublished in: ChemMedChem (2021)
To understand the potential in vitro modes of action of bis(β-diketonato) oxovanadium(IV) complexes, nine compounds of varying functionality have been screened using a range of biological techniques. The antiproliferative activity against a range of cancerous and normal cell lines has been determined, and show these complexes are particularly sensitive against the lung carcinoma cell line, A549. Annexin V (apoptosis) and Caspase-3/7 assays were studied to confirm these complexes induce programmed cell death. While gel electrophoresis was used to determine DNA cleavage activity and production of reactive oxygen species (ROS), the Comet assay was used to determine induced genomic DNA damage. Additionally, Förster resonance energy transfer (FRET)-based DNA melting and fluorescent intercalation displacement assays have been used to determine the interaction of the complexes with double strand (DS) DNA and to establish preferential DNA base-pair binding (AT versus GC).
Keyphrases
- energy transfer
- circulating tumor
- single molecule
- quantum dots
- dna damage
- cell free
- reactive oxygen species
- cell death
- oxidative stress
- high throughput
- nucleic acid
- living cells
- ionic liquid
- high resolution
- endoplasmic reticulum stress
- dna repair
- diabetic rats
- dna binding
- human health
- dna methylation
- mass spectrometry
- signaling pathway