Login / Signup

RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes.

Hui MaTao LiXuefeng XieLong JiangJingwei YeChenjia GongHanwei JiangSuixing FanHuan ZhangBaolu ShiBeibei ZhangXiaohua JiangYang LiJianteng ZhouJianze XuXingxia ZhangXiaoning HouHao YinYuanwei ZhangQinghua Shi
Published in: Science advances (2022)
Faithful segregation of X and Y chromosomes requires meiotic recombination to form a crossover between them in the pseudoautosomal region (PAR). Unlike autosomes that have approximately 10-fold more double-strand breaks (DSBs) than crossovers, one crossover must be formed from the one or two DSBs in PARs, implying the existence of a sex chromosome–specific recombination mechanism. Here, we found that RAD51AP2, a meiosis-specific partner of RAD51, is specifically required for the crossover formation on the XY chromosomes, but not autosomes. The decreased crossover formation between X and Y chromosomes in Rad51ap2 mutant mice results from compromised DSB repair in PARs due to destabilization of recombination intermediates rather than defects in DSB generation or synapsis. Our findings provide direct experimental evidence that XY recombination may use a PAR-specific DSB repair mechanism mediated by factors that are not essential for recombination on autosomes.
Keyphrases
  • dna repair
  • dna damage
  • open label
  • transcription factor
  • double blind
  • placebo controlled
  • oxidative stress
  • type diabetes
  • study protocol
  • metabolic syndrome
  • insulin resistance
  • genome wide