Loliolide in Sargassum horneri Alleviates Ultrafine Urban Particulate Matter (PM 0.1)-Induced Inflammation in Human RPE Cells.
Eun Jeoung LeeSol LeeHyun-Jae JangWonbeak YooPublished in: International journal of molecular sciences (2023)
Owing to increasing air pollution due to industrial development, fine dust has been associated with threatening public health. In particular, ultrafine urban particulate matter (uf-UP, PM 0.1) can easily enter our bodies, causing inflammation-related diseases. Therefore, in the present study, we evaluated the effects of hydrothermal extracts of Sargassum horneri and its bioactive compound, loliolide, on uf-UP-induced inflammation as a potential treatment strategy for retinal disorders. Human retinal pigment epithelial cells (ARPE-19) stimulated with TNF-α or uf-UPs were treated with S. horneri extract and loliolide. S. horneri extracts exhibited anti-inflammatory effects on uf-UP-induced inflammation without cell toxicity through downregulating the mRNA expression of MCP-1 , IL-8 , IL-6 , and TNF-α . UPLC-QTOF/MS analysis confirmed that the hydrothermal extract of S. horneri contained loliolide, which has anti-inflammatory effects. Loliolide effectively reduced the mRNA expression and production of proinflammatory chemokines ( IL-8 ) and cytokines ( IL-1β and IL-6 ) by downregulating the MAPK/NF-ĸB signaling pathway on TNF-α-stimulated inflammatory ARPE-19 cells. These effects were further confirmed in inflammatory ARPE-19 cells after stimulation with uf-UPs. Collectively, these results suggested the application of S. horneri as a functional ingredient for treating ocular disorders caused by particular matters.
Keyphrases
- particulate matter
- oxidative stress
- air pollution
- induced apoptosis
- diabetic rats
- signaling pathway
- endothelial cells
- high glucose
- public health
- cell cycle arrest
- pi k akt
- lung function
- rheumatoid arthritis
- anti inflammatory
- endoplasmic reticulum stress
- ms ms
- cell death
- multiple sclerosis
- drug induced
- single cell
- immune response
- heavy metals
- high resolution
- epithelial mesenchymal transition
- wastewater treatment
- human health
- diabetic retinopathy
- mass spectrometry
- inflammatory response
- cell proliferation