Chemical Constituents of the Culture Broth of Panus rudis .

In our ongoing search for new secondary metabolites from fungal strains, one novel compound ( 1 ) and nine known compounds ( 2-10 ) were isolated from the EtOAc-soluble layer of the culture broth of Panus rudis . The culture broth of P. rudis was extracted in acetone and fractionated by solvent partition; column chromatography using silica gel, Sephadex LH-20, and Sephadex G-10; MPLC; and HPLC. The structures of isolated compounds were elucidated by one- and two-dimensional NMR and LC-ESI-mass measurements. One new compound, panepoxydiol ( 1 ), and nine known compounds, ( E )-3-(3-hydroxy-3-methylbut-1-en-1-yl)-7-oxabicyclo[4.1.0]hept-3-ene-2,5-diol ( 2 ), isopanepoxydone ( 3 ), neopanepoxydone ( 4 ), panepoxydone ( 5 ), panepophenanthrin ( 6 ), 4-hydroxy-2,2-dimethyl-6-methoxychromane ( 7 ), 6-hydroxy-2,2-dimethyl-3-chromen ( 8 ), 2,2-dimethyl-6-methoxychroman-4-one ( 9 ), 3,4-dihydroxy-2,2-dimethyl-6-methoxychromane ( 10 ), were isolated from the culture broth of P. rudis . This is the first report of isolation of a new compound panepoxydiol ( 1 ) and nine other chemical constituents ( 2 - 5 , 7 - 10 ) from the culture broth of P. rudis .