Challenging the status quo: A comparison of ion exchange chromatography with liquid chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry methods for the measurement of amino acids in human plasma.
Rachel S CarlingBenjamin Ac McDonaldDonna AustinDeborah BurdenJoana CorreiaJenny LeungBeverley MayersCatharine JohnPublished in: Annals of clinical biochemistry (2021)
The LC-MS assay demonstrated variable analytical performance and correlated poorly with ion exchange chromatography. Both LC-MS/MS assays demonstrated comparable analytical performance and reasonable correlation with ion exchange chromatography. They also confer practical advantages which cannot be realized by ion exchange chromatography, superior specificity and significantly faster analysis time, suggesting that ion exchange chromatography should no longer be described as the gold standard method for plasma amino acid analysis.
Keyphrases
- liquid chromatography
- mass spectrometry
- tandem mass spectrometry
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- high resolution mass spectrometry
- solid phase extraction
- amino acid
- high performance liquid chromatography
- gas chromatography
- high speed
- capillary electrophoresis
- high resolution
- high throughput
- ms ms