Dynamics of DNA damage-induced nuclear inclusions are regulated by SUMOylation of Btn2.
Arun KumarVeena MathewPeter C StirlingPublished in: Nature communications (2024)
Spatial compartmentalization is a key facet of protein quality control that serves to store disassembled or non-native proteins until triage to the refolding or degradation machinery can occur in a regulated manner. Yeast cells sequester nuclear proteins at intranuclear quality control bodies (INQ) in response to various stresses, although the regulation of this process remains poorly understood. Here we reveal the SUMO modification of the small heat shock protein Btn2 under DNA damage and place Btn2 SUMOylation in a pathway promoting protein clearance from INQ structures. Along with other chaperones, and degradation machinery, Btn2-SUMO promotes INQ clearance from cells recovering from genotoxic stress. These data link small heat shock protein post-translational modification to the regulation of protein sequestration in the yeast nucleus.
Keyphrases
- heat shock protein
- quality control
- dna damage
- induced apoptosis
- heat shock
- oxidative stress
- cell cycle arrest
- protein protein
- emergency department
- amino acid
- dna repair
- diabetic rats
- cell death
- electronic health record
- small molecule
- transcription factor
- saccharomyces cerevisiae
- machine learning
- dna methylation
- heat stress