2'-Fluoro-c-di-GMP as an oral vaccine adjuvant.
Jia LiRhonda Kuo LeeWangxue ChenHongbin YanPublished in: RSC advances (2019)
Bis-(3'-5')-cyclic dimeric 2'-deoxy-2'-fluoroguanosine monophosphate (2'-F-c-di-GMP) was synthesized through the modified H-phosphonate chemistry. Oral immunization of C57BL/6 mice with Helicobacter pylori cell-free sonicate extract adjuvanted with 2'-F-c-di-GMP led to the production of antigen-specific antibodies in feces and sera, and lowered bacterial counts in the stomach upon post-vaccination infections in immunized mice. Similarly, oral vaccination of BALB/c mice with flagillin proteins from Clostridium difficile and Listeria monocytogenes adjuvanted with 2'-F-c-di-GMP led to production of antigen-specific antibodies both systemically and mucosally. The adjuvanticity of 2'-F-c-di-GMP is associated with the enhanced induction of interferon γ. These results demonstrated the excellent oral adjuvanticity of 2'-F-c-di-GMP.
Keyphrases
- biofilm formation
- pseudomonas aeruginosa
- helicobacter pylori
- staphylococcus aureus
- candida albicans
- escherichia coli
- cell free
- clostridium difficile
- high fat diet induced
- listeria monocytogenes
- early stage
- wild type
- computed tomography
- immune response
- adipose tissue
- metabolic syndrome
- insulin resistance
- skeletal muscle
- ionic liquid
- drug discovery
- oxide nanoparticles
- circulating tumor cells