A Colonic Organoid Model Challenged with the Large Toxins of Clostridioides difficile TcdA and TcdB Exhibit Deregulated Tight Junction Proteins.

Martina Schneemann Lucas Heils Verena MoosFranziska WeißSusanne M Krug January Mikolaj WeinerDieter BeuleRalf Gerhard Jörg-Dieter Schulzke Roland Bücker

Published in: Toxins (2023)

Adult stem cell-derived human colonic organoid monolayers were applicable as a colon infection model for electrophysiological measurements. The TJ changes noted can explain the epithelial barrier dysfunction and diarrhea in patients, as well as increased entry of luminal antigens triggering inflammation.

Keyphrases

end stage renal disease
clostridium difficile
oxidative stress
endothelial cells
newly diagnosed
ejection fraction
chronic kidney disease
peritoneal dialysis
prognostic factors
blood brain barrier
patient reported outcomes
induced pluripotent stem cells
patient reported