Whole-Cell cAMP and PKA Activity are Epiphenomena, Nanodomain Signaling Matters.
Donald M BersYang K XiangManuela ZaccoloPublished in: Physiology (Bethesda, Md.) (2020)
Novel targeted fluorescent biosensors provide key insights into very local nanodomains of cAMP and PKA activity, and how they respond differently to β-adrenergic activation in cardiac myocytes. This unique spatiotemporal detail in living cells is not available with biochemical measurements of total cellular cAMP and PKA, and provides unique physiological insights.