Highly Sensitive Voltammetric Glucose Biosensor Based on Glucose Oxidase Encapsulated in a Chitosan/Kappa-Carrageenan/Gold Nanoparticle Bionanocomposite.
Ilhem RassasMohamed BraiekAnne BonhommeFrancois BessueilleGuy RaffinHatem MajdoubNicole Jaffrezic-RenaultPublished in: Sensors (Basel, Switzerland) (2019)
In this work, an enzymatic sensor, based on a bionanocomposite film consisting of a polyelectrolyte complex (PEC) (Chitosan/kappa-carrageenan) doped with gold nanoparticles (AuNPs) encapsulating glucose oxidase (GOD) deposited on a gold electrode (Au) for glucose sensing, is described. Using the electrocatalytic synergy of AuNPs and GOD as a model of enzyme, the variation of the current (µA) as a function of the log of the glucose concentration (log [glucose]), shows three times higher sensitivity for the modified electrode (283.9) compared to that of the PEC/GOD modified electrode (93.7), with a detection limit of about 5 µM and a linearity range between 10 µM and 7 mM. The response of the PEC/AuNPs/GOD based biosensor also presents good reproducibility, stability, and negligible interfering effects from ascorbic acid, uric acid, urea, and creatinine. The applicability of the PEC/AuNPs/GOD based biosensor was tested in glucose-spiked saliva samples and acceptable recovery rates were obtained.
Keyphrases
- gold nanoparticles
- blood glucose
- uric acid
- reduced graphene oxide
- sensitive detection
- quantum dots
- label free
- drug delivery
- metabolic syndrome
- nuclear factor
- type diabetes
- blood pressure
- nitric oxide
- toll like receptor
- adipose tissue
- high resolution
- skeletal muscle
- inflammatory response
- loop mediated isothermal amplification
- solid state