A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu.
Juan ZhangQingqing LuQin DingLihong YinYuepu PuPublished in: International journal of environmental research and public health (2017)
A native, highly efficient microcystin-LR (MC-LR)-degrading bacterium named a7 was isolated from Lake Taihu and identified as Sphingopyxis sp. by 16S rDNA sequence analysis. The strain a7 could totally degrade MC-LR at a rate of 3.33 mg/(L•h), as detected by high-performance liquid chromatography (HPLC). The mlrA, mlrC, and mlrD genes were detected in the strain a7 by sequence analysis. Tetrapeptide and Adda-which are the middle metabolites of MC-LR-were analyzed via liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) during degradation. These metabolites were degraded completely, which suggested that the native Sphingopyxis sp. a7 was highly efficient in MC-LR degradation under bench conditions. Thus, strain a7 exhibited a significant potential application for bioremediation in water bodies contaminated by MC-LR produced by harmful cyanobacterial blooms.
Keyphrases
- highly efficient
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- ms ms
- mass spectrometry
- liquid chromatography
- solid phase extraction
- heavy metals
- drinking water
- gene expression
- gas chromatography
- genome wide
- high resolution
- dna methylation
- risk assessment
- human health
- transcription factor
- data analysis