Tissue dissociation for single-cell and single-nuclei RNA sequencing for low amounts of input material.
Gordon WieglebSusanne ReinhardtAndreas DahlNico PosnienPublished in: Frontiers in zoology (2022)
We present two dissociation protocols that allow isolating single cells and single nuclei, respectively, from low input material. Both protocols resulted in extraction of high-quality RNA for subsequent scRNAseq or snRNAseq applications. If tissue availability is limited, we recommend the snRNAseq procedure of fresh or frozen tissue samples as it is perfectly suited to obtain thorough insights into cellular diversity of complex tissue.