Coagulation-Inspired Direct Fibrinogen Assay Using Plasmonic Nanoparticles Functionalized with Red Blood Cell Membranes.
Insu KimDongtak LeeSang Won LeeJeong Hoon LeeGyudo LeeDae Sung YoonPublished in: ACS nano (2021)
The fast measurement of fibrinogen is essential in evaluating life-threatening sepsis and cardiovascular diseases. Here, we aim to utilize biomimetic plasmonic Au nanoparticles using red blood cell membranes (RBCM-AuNPs) and demonstrate nanoscale coagulation-inspired fibrinogen detection via cross-linking between RBCM-AuNPs. The proposed biomimetic RBCM-AuNPs are highly suitable for fibrinogen detection because hemagglutination, occurring in the presence of fibrinogen, induces a shift in the localized surface plasmon resonance of the NPs. Specifically, when the two ends of the fibrinogen protein are bound to receptors on separate RBCM-AuNPs, cross-linking of the RBCM-AuNPs occurs, yielding a corresponding plasmon shift within 10 min. This coagulation-inspired fibrinogen detection method, with a low sample volume, high selectivity, and high speed, could facilitate the diagnosis of sepsis and cardiovascular diseases.
Keyphrases
- red blood cell
- cardiovascular disease
- high speed
- label free
- intensive care unit
- loop mediated isothermal amplification
- acute kidney injury
- atomic force microscopy
- real time pcr
- mass spectrometry
- type diabetes
- single molecule
- coronary artery disease
- septic shock
- metabolic syndrome
- sensitive detection
- quantum dots
- cardiovascular events
- single cell
- walled carbon nanotubes