Exploring Peptaibol's Profile, Antifungal, and Antitumor Activity of Emericellipsin A of Emericellopsis Species from Soda and Saline Soils.
Anastasia E KuvarinaIrina A GavryushinaMaxim A SykonnikovTatiana A EfimenkoNatalia N MarkelovaElena N BilanenkoSofiya A BondarenkoLyudmila Y KokaevaAlla V TimofeevaMarina V SerebryakovaAnna S BarashkovaEugene A RogozhinMarina L GeorgievaVera S SadykovaPublished in: Molecules (Basel, Switzerland) (2022)
Features of the biochemical adaptations of alkaliphilic fungi to exist in extreme environments could promote the production of active antibiotic compounds with the potential to control microorganisms, causing infections associated with health care. Thirty-eight alkaliphilic and alkalitolerant Emericellopsis strains ( E. alkalina , E.  cf.  maritima , E.  cf.  terricola , Emericellopsis  sp.) isolated from different saline soda soils and belonging to marine, terrestrial, and soda soil ecological clades were investigated for emericellipsin A (EmiA) biosynthesis, an antifungal peptaibol previously described for Emericellopsis   alkalina . The analysis of the Emericellopsis sp. strains belonging to marine and terrestrial clades from chloride soils revealed another novel form with a mass of 1032.7 Da, defined by MALDI-TOF Ms/Ms spectrometers, as the EmiA lacked a hydroxyl (dEmiA). EmiA displayed strong inhibitory effects on cell proliferation and viability of HCT 116 cells in a dose- and time-dependent manners and induced apoptosis.
Keyphrases
- protein kinase
- induced apoptosis
- human health
- heavy metals
- endoplasmic reticulum stress
- cell proliferation
- healthcare
- signaling pathway
- mass spectrometry
- cystic fibrosis
- escherichia coli
- risk assessment
- climate change
- candida albicans
- cell cycle arrest
- single cell
- cell cycle
- high resolution
- cell death
- social media
- high speed