A universal DNA aptamer as an efficient inhibitor against spike-protein/hACE2 interactions.

Achut Prasad SilwalRaunak JahanSiddhartha Kalpa Samadhi ThennakoonSatya Prakash AryaRick Mason PostemaElizabeth Claire Vander ArkXiaohong Tan

Published in: Chemical communications (Cambridge, England) (2022)

A universal aptamer against spike-proteins of diverse SARS-CoV-2 variants was discovered via DNA SELEX towards the wild-type (WT) spike-protein. This aptamer, A1C1, binds to the WT spike-protein or other variants of concern such as Delta and Omicron with low nanomolar affinities. A1C1 inhibited the interaction between hACE2 and various spike-proteins by 85-89%. This universal A1C1 aptamer can be used to design diagnostic and therapeutic molecular tools to target SARS-CoV-2 and its variants.

Keyphrases

sars cov
gold nanoparticles
sensitive detection
copy number
wild type
magnetic nanoparticles
single molecule
circulating tumor
amino acid
respiratory syndrome coronavirus
label free
binding protein
cell free
gene expression
coronavirus disease
genome wide