In Situ Formed Catalytic Interface for Boosting Chemiluminescence.

Designing the catalytic interface that preferentially attracts reactants is highly desirable for amplifying chemiluminescence (CL) emission. Herein, to boost the generation of reactive oxygen species (ROS) from dissolved O2 molecule, flower-like cobalt hydroxide (f-Co(OH)2) based catalytic interface with hierarchical and porous architecture were in situ created in the coexistence of BSA and Co2+. Benefiting from the oxidase-like catalysis capability and the unique microstructure of f-Co(OH)2, ROS was efficiently produced. Meanwhile, the capping ligands of BSA endowed the interface with the capability of enriching functionality through the interaction between BSA and luminol. 100-fold CL enhancement was achieved using the as-prepared catalytic interface compared with the classical luminol-Co2+ or luminol-BSA system. Moreover, the proposed catalytic amplification mechanism could be extended to the different proteins such as lysozyme, protamine, thrombin, papain. Based on the quenching effect on CL, a sensitive sensing platform was constructed for the determination of ascorbic acid with satisfied results. Our finding provided a novel "all-in-one" route to design the catalytic interface for amplifying CL emission.