A new primer construction technique that effectively increases amplification of rare mutant templates in samples.

Jr-Kai HuangLing FanTao-Yeuan WangPao-Shu Wu

Published in: BMC biotechnology (2019)

In conclusion, our technique, stuntmer PCR, can provide a simple, low-cost, highly sensitive, highly accurate, and highly specific platform for developing companion diagnostic tests.

Keyphrases

low cost
high resolution
high throughput
nucleic acid
mass spectrometry
molecularly imprinted
real time pcr
single molecule