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Whole-Genome Sequencing of hMPXV1 in five Italian cases confirms the occurrence of the predominant epidemic lineage.

Diego ForniChiara MoltrasioManuela SironiAlessandra MozziEleonora QuattriLuigia VenegoniMarzia ZamprognoAndrea CitterioMario ClericiAngelo Valerio MarzanoRachele Cagliani
Published in: Journal of medical virology (2023)
The ongoing outbreak of monkeypox virus (hMPXV1) is the largest recorded in historically non-endemic countries. Genomic surveillance has emerged as a pivotal tool to track the spread and monitor the evolution of viral pathogens. Therefore, to assess the genetic diversity of circulating hMPXV1 in northern Italy in June-July 2022, we sequenced and analyzed five complete genomes of viruses sampled from patients presenting with a typical course of hMPXV1 infection. Phylogenetic analysis confirmed that all five genomes belong to the predominant epidemic lineage (B.1). Inspection of genetic changes and comparison with the reference sequence showed the presence of twelve nucleotide substitutions. Seven are non-synonymous mutations leading to amino acid changes in six proteins belonging to different functional classes. Moreover, eleven of these twelve nucleotide mutations involve GA>AA or TC >TT replacements, suggesting that host APOBEC3 enzymes are responsible for the generation of substitutions in circulating viruses. Finally, metagenomic analysis evidenced bacterial superinfection (Streptococcus pyogenes) in one patient. Through this study, we contributed to expand the number of complete genomes of viruses circulating in Italy and characterize them as belonging to the predominant outbreak lineage. This article is protected by copyright. All rights reserved.
Keyphrases
  • genetic diversity
  • amino acid
  • single cell
  • pet ct
  • public health
  • copy number
  • risk assessment
  • sars cov
  • case report
  • escherichia coli
  • dna methylation
  • antimicrobial resistance
  • wastewater treatment
  • staphylococcus aureus