Manganese Mineralization of Pathogenic Viruses as a Universal Vaccine Platform.
Pan-Deng ShiYan-Peng XuZhu ZhuChao ZhouMei WuYangzhige HeHui ZhaoLiying LiuLinqing ZhaoXiao-Feng LiCheng-Feng QinPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2023)
Biomimetic viral mineralization improves viral vaccine stability and immunogenicity using inorganic metals such as Ca, Al, or Fe. Mn is a metal found in high concentrations in mammalian tissues; however, under natural or laboratory conditions, Mn mineralization by medical viruses has yet to be established. Herein, a single IAV particle is successfully encapsulated with manganese phosphate (MnP) under specific conditions using the human influenza A virus (IAV). MnP-mineralized IAVs (IAV@Mn) exhibited physiochemical and in vitro properties similar to Ca-mineralized IAVs. In animal models, IAV@Mn shows limited replication in immune-competent cells and a significant attenuation compared to naïve cells. Moreover, a single-dose vaccination with IAV@Mn induced robust humoral and cellular immune responses and conferred significant protection against a wild-type IAV challenge in mice. Thus, Mn mineralization in pathogenic viruses provides a rapid and universal strategy for generating an emergency vaccine in response to emerging viruses.
Keyphrases
- immune response
- room temperature
- metal organic framework
- induced apoptosis
- transition metal
- wild type
- cell cycle arrest
- sars cov
- emergency department
- public health
- oxidative stress
- dendritic cells
- diabetic rats
- inflammatory response
- high glucose
- toll like receptor
- drug induced
- bone regeneration
- high fat diet induced
- heavy metals
- pi k akt