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Monitoring and modulating a catalytic hybridization circuit for self-adaptive bioorthogonal DNA assembly.

Xue GongShizhen HeRuomeng LiYingying ChenKaiyue TanYeqing WanJinghong LiFuan Wang
Published in: Chemical science (2022)
Constructing artificial domino nanoarchitectures, especially dynamic DNA circuits associated with the actuation of biological functions inside live cells, represents a versatile and powerful strategy to regulate the behaviors and fate of various living entities. However, the stepwise operation of conventional DNA circuits always relies on freely diffusing reactants, which substantially slows down their operation rate and efficiency. Herein, a self-adaptive localized catalytic circuit (LCC) is developed to execute the self-sustained bioorthogonal assembly of DNA nanosponges within a crowded intracellular environment. The LCC-generated DNA scaffolds are utilized as versatile templates for realizing the proximity confinement of LCC reactants. Single-molecule-detecting fluorescence correlation spectroscopy (FCS) is used to explore the reaction acceleration of the catalytic circuit. This self-adaptive DNA circuit facilitates the bioorthogonal assembly of highly branched DNA networks for robust and accurate monitoring of miRNA targets. Based on its intriguing and modular design, the LCC system provides a pivotal molecular toolbox for future applications in early disease diagnosis.
Keyphrases
  • single molecule
  • circulating tumor
  • atomic force microscopy
  • cell free
  • living cells
  • signaling pathway
  • current status
  • induced apoptosis
  • cell death
  • oxidative stress
  • mass spectrometry
  • crystal structure