Molecular and Structural Characterization of a Novel Escherichia coli Interleukin Receptor Mimic Protein.
Danilo G MorielBegoña HerasJason J PaxmanAlvin W LoLendl TanMatthew J SullivanSamantha J DandoScott A BeatsonGlen C UlettMark A SchembriPublished in: mBio (2016)
Uropathogenic E. coli (UPEC) is the primary cause of urinary tract infection (UTI), a disease of major significance to human health. Globally, the incidence of UPEC-mediated UTI is strongly associated with increasing antibiotic resistance, making this extremely common infection a major public health concern. In this report, we describe the regulatory, structural, functional, and immunogenic properties of a candidate UPEC vaccine antigen, IrmA. We demonstrate that IrmA is a small UPEC protein that forms a unique domain-swapped dimer with structural mimicry to several human cytokine receptors. We also show that IrmA binds to IL-2, IL-4, and IL-10, is strongly immunogenic in urosepsis patients, and is coexpressed with factors associated with biofilm formation. Overall, this work suggests a potential novel contribution for IrmA in UPEC infection.
Keyphrases
- escherichia coli
- urinary tract infection
- biofilm formation
- human health
- public health
- risk assessment
- pseudomonas aeruginosa
- staphylococcus aureus
- end stage renal disease
- candida albicans
- endothelial cells
- ejection fraction
- newly diagnosed
- binding protein
- peritoneal dialysis
- amino acid
- klebsiella pneumoniae
- risk factors
- multidrug resistant
- patient reported outcomes
- cystic fibrosis
- small molecule
- patient reported