Development and validation of a methodology for quantitative determination of malondialdehyde by HPLC-MC/MS.
P Yu MylnikovAleksey V ShchulkinYuliya Vladimirovna AbalenikhinaE N YakushevaPublished in: Klinicheskaia laboratornaia diagnostika (2022)
A bioanalytical technique for quantitative determination of MDA by HPLC-MS/MS. The proposed method for determining MDA includes the release stage of bound MDA and excludes the derivatization reaction. The lower limit of quantitative detection was 600 nmol/l, the volume of the required sample was 10 µl, the analysis time was 7 min. The range of concentrations obtained during the study makes it possible to use this bioanalytical technique to determine the concentration of MDA in biological material when assessing physiological and pathological conditions.
Keyphrases
- ms ms
- breast cancer cells
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- high performance liquid chromatography
- simultaneous determination
- cell cycle arrest
- high resolution
- molecularly imprinted
- mass spectrometry
- ultra high performance liquid chromatography
- gas chromatography mass spectrometry
- liquid chromatography
- multiple sclerosis
- cell death
- signaling pathway
- pi k akt
- label free
- gas chromatography
- quantum dots