Directed evolution approaches for optogenetic tool development.
Jaewan JangG Andrew WoolleyPublished in: Biochemical Society transactions (2021)
Photoswitchable proteins enable specific molecular events occurring in complex biological settings to be probed in a rapid and reversible fashion. Recent progress in the development of photoswitchable proteins as components of optogenetic tools has been greatly facilitated by directed evolution approaches in vitro, in bacteria, or in yeast. We review these developments and suggest future directions for this rapidly advancing field.