High efficiency penetration of antibody-immobilized nanoneedle thorough plasma membrane for in situ detection of cytoskeletal proteins in living cells.
R KawamuraK ShimizuY MatsumotoA YamagishiY R SilberbergM IijimaS KurodaK FukazawaK IshiharaChikashi NakamuraPublished in: Journal of nanobiotechnology (2016)
Operation at a lower temperature of 4 °C greatly improved the success rate of nanoneedle insertion to live cells at an optimized approach velocity, while it did not affect the binding of antibodies immobilized on the nanoneedle to vimentins for mechanical detection. As these experimental parameters can be applied to various cell types, these results may improve the versatility of the nanoneedle technology to other cell lines and platforms.
Keyphrases
- living cells
- high efficiency
- fluorescent probe
- loop mediated isothermal amplification
- induced apoptosis
- real time pcr
- single molecule
- ionic liquid
- cell cycle arrest
- single cell
- cell therapy
- stem cells
- oxidative stress
- signaling pathway
- endoplasmic reticulum stress
- transcription factor
- cell proliferation
- dna binding
- bone marrow
- sensitive detection