NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles.
Antonio BuonerbaRosita LapentaAnna DonniacuoMagda LicasaleElena VezzoliStefano MilioneCarmine CapacchioneMario Felice TecceAndrea FalquiRoberto PiacentiniClaudio GrassiAlfonso GrassiPublished in: Scientific reports (2020)
Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.
Keyphrases
- energy transfer
- gold nanoparticles
- photodynamic therapy
- cancer therapy
- drug release
- quantum dots
- drug delivery
- cell death
- fluorescent probe
- fluorescence imaging
- living cells
- reduced graphene oxide
- single cell
- radiofrequency ablation
- gene expression
- small molecule
- heat stress
- single molecule
- radiation therapy
- high resolution
- mass spectrometry
- cell proliferation
- liquid chromatography
- monte carlo